Molecular Evidence of Breast Cancer Cell Proliferation Inhibition by a Combination of Selected Qatari Medicinal Plants Crude Extracts
Abstract
: Breast cancer (BC) is the most common malignancy, and conventional medicine has failed
to establish efficient treatment modalities. Conventional medicine failed due to lack of knowledge of
the mechanisms that underpin the onset and metastasis of tumors, as well as resistance to treatment
regimen. However, Complementary and Alternative medicine (CAM) modalities are currently
drawing the attention of both the public and health professionals. Our study examined the effect of a
super-combination (SC) of crude extracts, which were isolated from three selected Qatari medicinal
plants, on the proliferation, motility and death of BC cells. Our results revealed that SC attenuated
cell growth and caused the cell death of MDA-MB-231 cancer cells when compared to human
normal neonatal fibroblast cells. On the other hand, functional assays showed that SC reduced BC
cell migration and invasion, respectively. SC-inhibited cell cycle and SC-regulated apoptosis was
most likely mediated by p53/p21 pathway and p53-regulated Bax/BCL-2/Caspace-3 pathway. Our
ongoing experiments aim to validate these in vitro findings in vivo using a BC-Xenograft mouse
model. These findings support our hypothesis that SC inhibited BC cell proliferation and induced
apoptosis. These findings lay the foundation for further experiments, aiming to validate SC as
an effective chemoprevention and/or chemotherapeutic strategy that can ultimately pave the way
towards translational research/clinical trials for the eradication of BC.
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