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AuthorNoorizadeh, Mohammad
AuthorGeetha, Mithra
AuthorBensaali, Faycal
AuthorMeskin, Nader
AuthorSadasivuni, Kishor K.
AuthorZughaier, Susu M.
AuthorElgamal, Mahmoud
AuthorAit Hssain, Ali
Available date2024-04-24T09:53:24Z
Publication Date2024
Publication NameBiosensors
ResourceScopus
ISSN20796374
URIhttp://dx.doi.org/10.3390/bios14010048
URIhttp://hdl.handle.net/10576/54211
AbstractIn response to the urgent requirement for rapid, precise, and cost-effective detection in intensive care units (ICUs) for ventilated patients, as well as the need to overcome the limitations of traditional detection methods, researchers have turned their attention towards advancing novel technologies. Among these, biosensors have emerged as a reliable platform for achieving accurate and early diagnoses. In this study, we explore the possibility of using Pyocyanin analysis for early detection of pathogens in ventilator-associated pneumonia (VAP) and lower respiratory tract infections in ventilated patients. To achieve this, we developed an electrochemical sensor utilizing a graphene oxide-copper oxide-doped MgO (GO - Cu - Mgo) (GCM) catalyst for Pyocyanin detection. Pyocyanin is a virulence factor in the phenazine group that is produced by Pseudomonas aeruginosa strains, leading to infections such as pneumonia, urinary tract infections, and cystic fibrosis. We additionally investigated the use of DNA aptamers for detecting Pyocyanin as a biomarker of Pseudomonas aeruginosa, a common causative agent of VAP. The results of this study indicated that electrochemical detection of Pyocyanin using a GCM catalyst shows promising potential for various applications, including clinical diagnostics and drug discovery.
SponsorThis paper was supported by an International Research Collaboration Co-Fund (IRCC) grant of Qatar University under grant no. IRCC-2022-569. This work was additionally supported by the Qatar National Research Fund under grant no. MME03-1226-210042. The findings achieved and statements made herein are solely the responsibility of the authors.
Languageen
PublisherMultidisciplinary Digital Publishing Institute (MDPI)
Subjectbio-signal processing
biosensors
DNA aptamer
electrochemistry
nosocomial infections
ventilator-associated pneumonia (VAP)
TitleA Path towards Timely VAP Diagnosis: Proof-of-Concept Study on Pyocyanin Sensing with Cu-Mg Doped Graphene Oxide
TypeArticle
Issue Number1
Volume Number14
dc.accessType Open Access


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