Elaeagnus angustifolia Plant Extract Inhibits Epithelial-Mesenchymal Transition and Induces Apoptosis via HER2 Inactivation and JNK Pathway in HER2-Positive Breast Cancer Cells
Author | Jabeen, Ayesha |
Author | Sharma, Anju |
Author | Gupta, Ishita |
Author | Kheraldine, Hadeel |
Author | Vranic, Semir |
Author | Al Moustafa, Ala-Eddin |
Author | Al Farsi, Halema F |
Available date | 2020-09-22T10:22:54Z |
Publication Date | 2020-09-16 |
Publication Name | Molecules |
Identifier | http://dx.doi.org/10.3390/molecules25184240 |
Citation | Jabeen, A.; Sharma, A.; Gupta, I.; Kheraldine, H.; Vranic, S.; Al Moustafa, A.-E.; Al Farsi, H.F. Elaeagnus angustifolia Plant Extract Inhibits Epithelial-Mesenchymal Transition and Induces Apoptosis via HER2 Inactivation and JNK Pathway in HER2-Positive Breast Cancer Cells. Molecules 2020, 25, 4240. https://doi.org/10.3390/molecules25184240 |
Abstract | Elaeagnus angustifolia (EA) is a medicinal plant used for treating several human diseases in the Middle East. Meanwhile, the outcome of extract on HER2-positive breast cancer remains nascent. Thus, we herein investigated the effects of the aqueous extract obtained from the flowers of on two HER2-positive breast cancer cell lines, SKBR3 and ZR75-1. Our data revealed that extract inhibits cell proliferation and deregulates cell-cycle progression of these two cancer cell lines. extract also prevents the progression of epithelial-mesenchymal transition (EMT), an important event for cancer invasion and metastasis; this is accompanied by upregulations of E-cadherin and β-catenin, in addition to downregulations of vimentin and fascin, which are major markers of EMT. Thus, extract causes a drastic decrease in cell invasion ability of SKBR3 and ZR75-1 cancer cells. Additionally, we found that extract inhibits colony formation of both cell lines in comparison with their matched control. The molecular pathway analysis of HER2 and JNK1/2/3 of extract exposed cells revealed that it can block HER2 and JNK1/2/3 activities, which could be the major molecular pathway behind these events. Our findings implicate that extract may possess chemo-preventive effects against HER2-positive breast cancer via HER2 inactivation and specifically JNK1/2/3 signaling pathways. |
Sponsor | grants from Qatar University: # QUCP-CMED-2019-1, QUHI-CMED-19/20-1, and QUCG-CMED-20/21-2. |
Language | en |
Publisher | MDPI |
Subject | EMT Elaeagnus angustifolia apoptosis breast cancer chemoprevention |
Type | Article |
Issue Number | 18 |
Volume Number | 25 |
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