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    Real-Time SARS-CoV-2 Genotyping by High-Throughput Multiplex PCR Reveals the Epidemiology of the Variants of Concern in Qatar

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    Real-Time SARS-CoV-2 Genotyping by High-Throughput Multiplex PCR Reveals the Epidemiology of the Variants of Concern in Qatar.pdf (630.2Kb)
    Date
    2021-11
    Author
    R. Hasan, Mohammad
    K.R. Kalikiri, Mahesh
    Mirza, Faheem
    Sundararaju, Sathyavathi
    Sharma, Anju
    Xaba, Thabisile
    Lorenz, Stephan
    Chemaitelly, Hiam
    A. El-Kahlout, Reham
    Ming Tsui, Kin
    M. Yassine, Hadi
    V. Coyle, Peter
    Al Khal, Abdullatif
    Bertollini, Roberto
    H. Al Thani, Mohamed
    J. Abu-Raddad, Laith
    Tang, Patrick
    ...show more authors ...show less authors
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    Abstract
    Complementing whole genome sequencing strategies with high-throughput multiplex RT-qPCR genotyping allows for more comprehensive and real-time tracking of SARS-CoV-2 variants of concern. During the second and third waves of COVID-19 in Qatar, PCR genotyping, combined with Sanger sequencing of un-typeable samples, was employed to describe the epidemiology of the Alpha, Beta and Delta variants. A total of 9792 nasopharyngeal PCR-positive samples collected between April-June 2021 were successfully genotyped, revealing the importation and transmission dynamics of these three variants in Qatar.
    DOI/handle
    http://dx.doi.org/10.1016/j.ijid.2021.09.006
    http://hdl.handle.net/10576/24827
    Collections
    • Biomedical Research Center Research [‎794‎ items ]
    • COVID-19 Research [‎848‎ items ]

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