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AuthorFouzat, Arij
AuthorHussein, Ola Jihad
AuthorGupta, Ishita
AuthorAl-Farsi, Halema F.
AuthorKhalil, Ashraf
AuthorAl Moustafa, Ala Eddin
Available date2022-10-24T07:36:13Z
Publication Date2022-03-18
Publication NameFrontiers in Nutrition
Identifierhttp://dx.doi.org/10.3389/fnut.2022.871667
CitationFouzat A, Hussein OJ, Gupta I, Al-Farsi HF, Khalil A and Al Moustafa A-E (2022) Elaeagnus angustifolia Plant Extract Induces Apoptosis via P53 and Signal Transducer and Activator of Transcription 3 Signaling Pathways in Triple-Negative Breast Cancer Cells. Front. Nutr. 9:871667. doi: 10.3389/fnut.2022.871667
URIhttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85127948358&origin=inward
URIhttp://hdl.handle.net/10576/35341
AbstractElaeagnus angustifolia (EA) is used as an alternative medicine in the Middle East to manage numerous human diseases. We recently reported that EA flower extract inhibits cell proliferation and invasion of human oral and HER2-positive breast cancer cells. Nevertheless, the outcome of EA extract on triple-negative breast cancer (TNBC) cells has not been explored yet. We herein investigate the effect of the aqueous EA extract (100 and 200 μl/ml) on two TNBC cell lines (MDA-MB-231 and MDA-MB-436) for 48 h and explore its underlying molecular pathways. Our data revealed that EA extract suppresses cell proliferation by approximately 50% and alters cell-cycle progression of these two cancer cell lines. Additionally, EA extract induces cell apoptosis by 40–50%, accompanied by the upregulation of pro-apoptotic markers (Bax and cleaved caspase-8) and downregulation of the anti-apoptotic marker, Bcl-2. Moreover, EA extract inhibits colony formation compared to their matched control. More significantly, the molecular pathway analysis of EA-treated cells revealed that EA extract enhances p53 expression, while inhibiting the expression of total and phosphorylated Signal Transducer and Activator Of Transcription 3 (STAT3) in both cell lines, suggesting p53 and STAT3 are the main key players behind the biological events provoked by the extract in TNBC cells. Our findings implicate that EA flower extract may possess an important potential as an anticancer drug against TNBC.
SponsorGrants from Qatar University: # QUST-1-CPH-2021-22, QUCP-CMED-22/23-529, and QUCG-CMED-20/21-2.
Languageen
PublisherFrontiers Media
Subjectapoptosis
Elaeagnus angustifolia
p53
stat3
triple-negative breast cancer
TitleElaeagnus angustifolia Plant Extract Induces Apoptosis via P53 and Signal Transducer and Activator of Transcription 3 Signaling Pathways in Triple-Negative Breast Cancer Cells
TypeArticle
Pagination1-11
Volume Number9
ESSN2296-861X
dc.accessType Open Access


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