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AuthorHebah A., Al-Khatib
AuthorSmatti, Maria K.
AuthorAli, Fatma H.
AuthorZedan, Hadeel T.
AuthorThomas, Swapna
AuthorAhmed, Muna N.
AuthorEl-kahlout, Reham A.
AuthorAl Bader, Mashael A.
AuthorElgakhlab, Dina
AuthorCoyle, Peter V.
AuthorAbu-Raddad, Laith J.
AuthorAl Thani, Asma A.
AuthorYassine, Hadi M.
Available date2023-06-21T05:24:44Z
Publication Date2022-11-18
Publication NameiScience
Identifierhttp://dx.doi.org/10.1016/j.isci.2022.105438
ISSN25890042
URIhttps://www.sciencedirect.com/science/article/pii/S2589004222017102
URIhttp://hdl.handle.net/10576/44626
AbstractSevere acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a rapidly evolving RNA virus that mutates within hosts and exists as viral quasispecies. Here, we evaluated the within-host diversity among vaccinated and unvaccinated individuals (n = 379) infected with different SARS-CoV-2 Variants of Concern. The majority of samples harbored less than 14 intra-host single-nucleotide variants (iSNVs). A deep analysis revealed a significantly higher intra-host diversity in Omicron samples than in other variants (p value < 0.05). Vaccination status and type had a limited impact on intra-host diversity except for Beta-B.1.315 and Delta-B.1.617.2 vaccinees, who exhibited higher diversity than unvaccinated individuals (p values: <0.0001 and <0.0021, respectively). Three immune-escape mutations were identified: S255F in Delta and R346K and T376A in Omicron-B.1.1.529. The latter 2 mutations were fixed in BA.1 and BA.2 genomes, respectively. Overall, the relatively higher intra-host diversity among vaccinated individuals and the detection of immune-escape mutations, despite being rare, suggest a potential vaccine-induced immune pressure in vaccinated individuals.
SponsorThe authors are grateful for the leadership and assistance provided by the Ministry of Public Health in Qatar, the virology laboratory staff at Hamad Medical Corporation, and Qatar Biobank (QBB) team. This project was funded by Qatar National Research Fund (QNRF; Project number UREP28-164-3-048) and Qatar University (Project number QUCG-BRC-22/23-547). The article processing charges were paid from grant no. QUCG-BRC-2022/23-578.
Languageen
PublisherElsevier
SubjectImmunology
Immune response
Virology
Genomics
TitleComparative analysis of within-host diversity among vaccinated COVID-19 patients infected with different SARS-CoV-2 variants
TypeArticle
Issue Number11
Volume Number25
Open Access user License http://creativecommons.org/licenses/by/4.0/
dc.accessType Open Access


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