Detection and Genotyping of Torque Teno Virus (TTV) in Healthy Blood Donors and Patients Infected With HBV or HCV in Qatar
Date
2015-02Author
AbuOdeh, RaedAl-Mawlawi, Naema
Al-Qahtani, Ahmed
Bohol, Marie Fe F.
Al-Ahdal, Mohammed N.
Hasan, Haydar A.
AbuOdeh, Lamees
Nasrallah, Gheyath K.
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Metadata
Show full item recordAbstract
Torque Teno virus (TTV) has been associated
with non A–G hepatitis. The goal of this study
was to estimate the infection rates and genotypic
characteristics of TTV in the State of Qatar.
A total of 644 blood samples representing different
nationalities: (i) Qatari (118) and (ii) non-
Qatari (526) nationals (mostly from Arab and
South Eeast Asia countries) were tested for the
presence of TTV DNA by nested PCR. The
majority (573) of the blood samples belonged to
healthy blood donors, whereas 54 and 53 of the
blood samples belonged to patients infected
with hepatitis B virus (HBV) and hepatitis C virus
(HCV), respectively. The results obtained
showed that the TTV infection rates in the
healthy blood donors, and those infected with
HBV or HCV patients were 81.4, 90.75 and 84.9%,
respectively. Significant association between
TTV viremia and age, or nationality was observed.
Sequence analysis of PCR fragments
amplified from the 50-untranslated region (50-
UTR) of all (531) TTV positive samples showed
that 65.5% (348/531) of the PCR fragment sequences
were classified into main genogroup 3,
followed by main genogroups 5 (24%), 2 (5.8%),
and 1 (4.7%). Genogroup 4 was not detected
among the our studied subjects. Phylogenetic
and pairwise analyses using sequences from
TTV viremic samples also showed an overall
close similarity to the main genogroup 3. In conclusion,
there was no significant difference in
the rates of TTV detection among Qataris and
non-Qataris and several genotypes, mainly
genotype 3, were isolated.
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