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المؤلفThomas, Swapna
المؤلفSmatti, Maria K.
المؤلفAlsulaiti, Haya
المؤلفZedan, Hadeel T.
المؤلفEid, Ali H.
المؤلفHssain, Ali A.
المؤلفAbu Raddad, Laith J.
المؤلفGentilcore, Giusy
المؤلفOuhtit, Allal
المؤلفAlthani, Asmaa A.
المؤلفNasrallah, Gheyath K.
المؤلفGrivel, Jean Charles
المؤلفYassine, Hadi M.
تاريخ الإتاحة2024-05-23T06:59:49Z
تاريخ النشر2024-05-01
اسم المنشورJournal of Medical Virology
المعرّفhttp://dx.doi.org/10.1002/jmv.29628
الاقتباسThomas S, Smatti MK, Alsulaiti H, et al. Antibody-dependent enhancement (ADE) of SARS-CoV-2 in patients exposed to MERS-CoV and SARS-CoV-2 antigens. J Med Virol. 2024; 96:e29628. doi:10.1002/jmv.29628
الرقم المعياري الدولي للكتاب01466615
معرّف المصادر الموحدhttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85191705968&origin=inward
معرّف المصادر الموحدhttp://hdl.handle.net/10576/55333
الملخصThis study evaluated the potential for antibody-dependent enhancement (ADE) in serum samples from patients exposed to Middle East respiratory syndrome coronavirus (MERS-CoV). Furthermore, we evaluated the effect of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) vaccination on ADE in individuals with a MERS infection history. We performed ADE assay in sera from MERS recovered and SARS-CoV-2-vaccinated individuals using BHK cells expressing FcgRIIa, SARS-CoV-2, and MERS-CoV pseudoviruses (PVs). Further, we analyzed the association of ADE to serum IgG levels and neutralization. Out of 16 MERS patients, nine demonstrated ADE against SARS-CoV-2 PV, however, none of the samples demonstrated ADE against MERS-CoV PV. Furthermore, out of the seven patients exposed to SARS-CoV-2 vaccination after MERS-CoV infection, only one patient (acutely infected with MERS-CoV) showed ADE for SARS-CoV-2 PV. Further analysis indicated that IgG1, IgG2, and IgG3 against SARS-CoV-2 S1 and RBD subunits, IgG1 and IgG2 against the MERS-CoV S1 subunit, and serum neutralizing activity were low in ADE-positive samples. In summary, samples from MERS-CoV-infected patients exhibited ADE against SARS-CoV-2 and was significantly associated with low levels of neutralizing antibodies. Subsequent exposure to SARS-CoV-2 vaccination resulted in diminished ADE activity while the PV neutralization assay demonstrated a broadly reactive antibody response in some patient samples.
راعي المشروعWe thank Yoshihiro Kawaoka et al. for providing BHK cells expressing FcgRIIa. We also thank the Virology Research Center at the National Institutes of Health for providing the plasmids used to generate SARS-CoV-2 PVs and for providing HEK293T cells. This study was supported by partial funds from QU grant no. QUCG-BRC-2022/23-578.
اللغةen
الناشرWiley
الموضوعantibody-dependent enhancement
COVID-19
cross-reactive antibodies
FcgRIIa
MERS-CoV
non-neutralizing antibodies
SARS-CoV-2
العنوانAntibody-dependent enhancement (ADE) of SARS-CoV-2 in patients exposed to MERS-CoV and SARS-CoV-2 antigens
النوعArticle
رقم العدد5
رقم المجلد96
ESSN1096-9071
dc.accessType Open Access


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