Diagnostic Efficiency of Three Fully Automated Serology Assays and Their Correlation with a Novel Surrogate Virus Neutralization Test in Symptomatic and Asymptomatic SARS-COV-2 Individuals
Date
2020-01-25Author
Younes, SalmaAl-Jighefee, Hadeel
Shurrab, Farah
Al-Sadeq, Duaa W.
Younes, Nadin
Dargham, Soha R.
Al-Dewik, Nader
Qotba, Hamda
Syed, Mohamed
Alnuaimi, Ahmed
Yassine, Hadi M.
Tang, Patrick
Abu-Raddad, Laith J.
Nasrallah, Gheyath K.
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Metadata
Show full item recordAbstract
Abstract: To support the deployment of serology assays for population screening during the
COVID-19 pandemic, we compared the performance of three fully automated SARS-CoV-2 IgG assays: Mindray CL-900i® (target: spike [S] and nucleocapsid [N]), BioMérieux VIDAS®3 (target: receptor-binding domain [RBD]) and Diasorin LIAISON®XL (target: S1 and S2 subunits). A total of
111 SARS-CoV-2 RT-PCR- positive samples collected at ≥ 21 days post symptom onset, and 127 prepandemic control samples were included. Diagnostic performance was assessed in correlation to
RT-PCR and a surrogate virus-neutralizing test (sVNT). Moreover, cross-reactivity with other viral
antibodies was investigated. Compared to RT-PCR, LIAISON®XL showed the highest overall specificity (100%), followed by VIDAS®3 (98.4%) and CL-900i® (95.3%). The highest sensitivity was
demonstrated by CL-900i® (90.1%), followed by VIDAS®3 (88.3%) and LIAISON®XL (85.6%). The
sensitivity of all assays was higher in symptomatic patients (91.1–98.2%) compared to asymptomatic
patients (78.4–80.4%). In correlation to sVNT, all assays showed excellent sensitivities (92.2–96.1%). In addition, VIDAS®3 demonstrated the best correlation (r = 0.75) with the sVNT. The present study
provides insights on the performance of three fully automated assays, which could help diagnostic
laboratories in the choice of a particular assay according to the intended use
DOI/handle
http://dx.doi.org/10.3390/microorganisms9020245http://hdl.handle.net/10576/17467
http://dx.doi.org/10.3390/microorganisms9020245
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