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AuthorAlheidous, Sara
AuthorAl-Muraikhy, Shamma
AuthorRizk, Nasser
AuthorSellami, Maha
AuthorDonati, Francesco
AuthorBotre, Francesco
AuthorAl-Mansoori, Layla
AuthorElrayess, Mohamed A.
Available date2022-12-20T09:01:06Z
Publication Date2022-11-24
Publication NameFrontiers in Molecular Biosciences
Identifierhttp://dx.doi.org/10.3389/fmolb.2022.943034
CitationAlheidous S, Al-Muraikhy S, Rizk N, Sellami M, Donati F, Botre F, Al-Mansoori L and Elrayess MA (2022) Effect of sera from elite athletes on cytokine secretion and insulin signaling in preadipocytes and skeletal muscle cells. Front. Mol. Biosci. 9:943034. doi: 10.3389/fmolb.2022.943034
URIhttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85143398071&origin=inward
URIhttp://hdl.handle.net/10576/37421
AbstractIntroduction: The immunomodulatory effect of physical activity can impact insulin signaling differentially in adipose tissues and skeletal muscle cells, depending on sport intensity. In this study, the effect of serum from elite athletes with varying endurance levels and playing different power sports on cytokine secretion and insulin signaling in preadipocyte and skeletal muscle cell lines was investigated. Methods: Preadipocytes (3T3-L1) and skeletal muscle cells (C2C12) were cultured in media containing pooled sera from elite athletes who play high-endurance (HE), high-power (HP), or low-endurance/low-power (LE/LP) sports for 72 h. Secreted cytokines (IL-6 and TNF-alpha) were assessed in the supernatant, and insulin signaling phosphoproteins levels were measured in lysates following treatment using cells multiplex immunoassays. Results: Sera from LE/LP and HP induced TNF-α secretion in C2C12, while serum from HE reduced IL-6 secretion compared to non-athlete serum control. All elite athlete sera groups caused decreased insulin sensitivity in 3T3-L1 cells, whereas in C2C12 cells, only HE athlete serum reduced insulin signaling, while LE/LP and HP caused increased insulin sensitivity. Conclusion: Sera from elite athletes of different sport disciplines can affect the inflammatory status and insulin signaling of preadipocytes and myoblasts differently, with risk of developing insulin resistance. Furthermore, investigation of the functional relevance of these effects on exercise physiology and pathophysiology is warranted.
SponsorThis research was sponsored by the award no. QUCG-BRC-21/22-1 (ME and NR), IRCC-2022-467 (ME and MS) from Qatar University. The statements made herein are solely the responsibility of the authors.
Languageen
PublisherFrontiers Media
Subjectelite athletes
high-endurance sports
high-power sports
inflammation
insulin signaling
preadipocytes
skeletal muscle cells
TitleEffect of sera from elite athletes on cytokine secretion and insulin signaling in preadipocytes and skeletal muscle cells
TypeArticle
Volume Number9
ESSN2296-889X
dc.accessType Open Access


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