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المؤلفAbbas, Tariq O.
المؤلفParangusan, Hemalatha
المؤلفYalcin, Huseyin C.
المؤلفHassan, Mohamed
المؤلفZakrif, Lubna
المؤلفZandi, Nooshin
المؤلفPennisi, Cristian P.
تاريخ الإتاحة2024-04-22T06:41:47Z
تاريخ النشر2024-03-01
اسم المنشورBiomedical Materials (Bristol)
المعرّفhttp://dx.doi.org/10.1088/1748-605X/ad1c9c
الاقتباسTariq O Abbas et al 2024 Biomed. Mater. 19 025022 DOI 10.1088/1748-605X/ad1c9c
الرقم المعياري الدولي للكتاب17486041
معرّف المصادر الموحدhttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85183473489&origin=inward
معرّف المصادر الموحدhttp://hdl.handle.net/10576/54028
الملخصRegeneration of damaged urethral tissue remains a major challenge in the field of lower urinary tract reconstruction. To address this issue, various synthetic and natural biodegradable biomaterials are currently being explored for the fabrication of scaffolds that promote urethral regeneration and healing. In this study, we present an approach to fabricate a trilayer hybrid scaffold comprising a central layer of poly(lactic acid) (PLA) between two layers of chitosan. The chitosan/PLA/chitosan (CPC) scaffolds were fabricated by a sequential electrospinning process and their properties were evaluated for their suitability for urethral tissue engineering. The physical and biological properties of the CPC scaffolds were evaluated in comparison to electrospun PLA scaffolds and acellular dermis (Alloderm) as controls for a synthetic and a natural scaffold, respectively. Compared to the controls, the CPC scaffolds exhibited higher elastic modulus and ultimate tensile strength, while maintaining extensibility and suture retention strength appropriate for clinical use. The CPC scaffolds displayed significant hydrophilicity, which was associated with a higher water absorption capacity of the chitosan nanofibres. The degradation products of the CPC scaffolds did not exhibit cytotoxicity and promoted wound closure by fibroblasts in vitro. In addition, CPC scaffolds showed increased growth of smooth muscle cells, an essential component for functional regeneration of urethral tissue. Furthermore, in a chicken embryo-based assay, CPC scaffolds demonstrated significantly higher angiogenic potential, indicating their ability to promote vascularisation, a crucial aspect for successful urethral reconstruction. Overall, these results suggest that CPC hybrid scaffolds containing both natural and synthetic components offer significant advantages over conventional acellular or synthetic materials alone. CPC scaffolds show promise as potential candidates for further research into the reconstruction of the urethra in vivo.
اللغةen
الناشرIOP Publishing
الموضوعhybrid scaffolds, chitosan. angiogenesis
tissue engineering
urethral reconstruction
العنوانTrilayer composite scaffold for urethral reconstruction: in vitro evaluation of mechanical, biological, and angiogenic properties
النوعArticle
رقم العدد2
رقم المجلد19
ESSN1748-605X
dc.accessType Open Access


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